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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Ultralow voltage, OTFT-based sensor for label-free DNA detection.

S Lai¹, M Demelas, G Casula

¹Dipartimento di Ingegneria Elettrica ed Elettronica, Università di Cagliari, Italy.

Advanced Materials (Deerfield Beach, Fla.)

|October 3, 2012

Summary

This study introduces an organic ultralow voltage field effect transistor for label-free DNA hybridization detection. The novel device achieves sub-nanometer detection limits and high selectivity for single nucleotide polymorphism identification.

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Area of Science:

Organic electronics
Biosensors
Molecular diagnostics

Background:

DNA hybridization is crucial for molecular diagnostics.
Existing detection methods often require labels or are less sensitive.
Field-effect transistors offer potential for sensitive, label-free detection.

Purpose of the Study:

To develop and present an organic ultralow voltage field effect transistor (FET) for DNA hybridization detection.
To demonstrate label-free detection capabilities.
To evaluate the device's sensitivity and selectivity.

Main Methods:

Fabrication of an organic ultralow voltage field effect transistor.
Utilizing field-effect modulation by oligonucleotide charge for transduction.
Testing the device for DNA hybridization detection and single nucleotide polymorphism (SNP) analysis.

Main Results:

The organic FET achieved label-free detection of DNA hybridization.
Demonstrated a sub-nanometer detection limit.
Exhibited unprecedented selectivity for single nucleotide polymorphism detection.

Conclusions:

Organic ultralow voltage FETs are effective for sensitive and selective DNA hybridization detection.
The label-free approach simplifies the detection process.
This technology holds promise for advanced molecular diagnostics.