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Mitochondrial aspartate aminotransferase determined by "Fast Protein Liquid Chromatography"
1Institute of Medical Biology, University of Tromsø, Norway.
Clinical Chemistry
|February 1, 1990
Abstract:
We describe an improved separation of the isoenzymes of aspartate aminotransferase (EC 2.6.1.1), based on ion-exchange chromatography. Involving the "Fast Protein Liquid Chromatography" system (Pharmacia) with a MonoQ column, this rapid, reproducible method for quantifying the mitochondrial enzyme shows good resolution and sensitivity, and results correlate well with those by an established immunochemical method.