Related Experiment Video
Updated: May 18, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Kinetics of nitrite evaluated in a meat product
G Barbieri1, M Bergamaschi, Ge Barbieri
1Stazione Sperimentale per l'Industria delle Conserve Alimentari, 43100 Parma, Italy. giampiero.barbieri@ssica.it
Optimizing sodium nitrite levels and temperature in mortadella production is key. A specific combination ensures efficient nitrite reactions, preventing undesirable nitrate and nitric oxide buildup for product quality.
Area of Science:
- Food Science
- Meat Science
- Chemical Kinetics
Background:
- Sodium nitrite is crucial in processed meats like mortadella for color, flavor, and microbial safety.
- Understanding nitrite reaction kinetics is essential for optimizing its use and preventing adverse effects.
Purpose of the Study:
- To evaluate the efficiency of nitrite reactions in mortadella.
- To determine the effect of sodium nitrite concentration and temperature on reaction kinetics.
Main Methods:
- Kinetics parameters were calculated at varying sodium nitrite levels (40-150 mg/kg).
- Experiments were conducted at temperatures ranging from 55°C to 72°C.
- The study focused on color formation and nitrite reduction pathways.
Main Results:
- Color formation is favored by low activation energy.
- Optimal conditions for direct nitrite reduction were identified.
- A specific ingoing amount of sodium nitrite (70 mg/kg) and rapid heating to 65°C were found to be crucial.
Conclusions:
- Achieving 65°C rapidly with 70 mg/kg sodium nitrite is vital for efficient nitrite utilization in mortadella.
- This approach prevents excessive nitrate and nitric oxide formation.
- Optimized kinetics ensure product quality and safety.
Related Concept Videos
2° Amines to N-Nitrosamines: Reaction with NaNO2
Inorganic Nitrogen Assimilation
Nitrosation of Enols
Enzyme Kinetics
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by water loss...
Introduction to Enzyme Kinetics
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...

