Related Experiment Video
Updated: May 18, 2026

08:28
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
Published on: September 19, 2017
Kinetic template-guided tethering of fragments
Rebecca H Nonoo1, Alan Armstrong, David J Mann
1Department of Chemistry, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.
Chemmedchem
|October 4, 2012
Abstract:
Birds of a tether: A tethering strategy for the site-directed discovery of low-molecular-weight fragments that bind weakly to defined protein surfaces is described. A solvent-exposed protein thiol captures acrylamide-modified fragments in a conjugate addition reaction that requires a template to produce a measureable quantity of protein-fragment adduct, which can be rapidly identified by mass spectrometry.

