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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 18, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

The microarray technology: facts and controversies.

N Lěvěque¹, F Renois², L Andréoletti²

¹Clinical and Molecular Virology Unit, University Hospital; Clinical and Molecular Virology Unit, University Hospital.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases

|October 5, 2012

Summary

Molecular diagnostic techniques, like multiplex RT-PCR and microarray analysis, offer simultaneous detection of multiple viruses. This advancement aids in diagnosing viral infections, particularly in critical care settings.

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Last Updated: May 18, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

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Published on: March 15, 2011

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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Area of Science:

Clinical Virology
Molecular Diagnostics
Infectious Diseases

Background:

Classical virological assays are being increasingly replaced by advanced molecular diagnostic techniques.
Molecular methods offer enhanced sensitivity and specificity for viral detection.

Purpose of the Study:

To evaluate multiplex RT-PCR amplification followed by microarray analysis for simultaneous viral detection.
To assess the utility of microarrays in diagnosing viral central nervous system and respiratory tract infections.

Main Methods:

Multiplex reverse transcription-polymerase chain reaction (RT-PCR) amplification of viral genome-specific fragments.
Hybridization of amplified fragments with microorganism-specific probes on solid surfaces (microarrays).
Simultaneous detection and identification of multiple viruses in single clinical samples.

Main Results:

Microarrays demonstrate reliable performance comparable to reference RT-PCR assays.
Successful application in diagnosing viral central nervous system and respiratory tract infections.
Potential for detecting a broad range of viruses, including novel and neglected ones.

Conclusions:

Microarray technology shows significant promise for improving the clinical management of viral infections.
Enhanced viral detection capabilities can benefit critically ill patients and aid in preventing nosocomial transmission.
Development of new epidemiological surveillance systems for viral infections is facilitated.