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Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
Published on: August 2, 2018
Improved signal model for confocal sensors accounting for object depending artifacts
Florian Mauch1, Wolfram Lyda, Marc Gronle
1Institute for Technical Optics, Stuttgart Research Centre of Photonic Engineering (SCoPE), University Stuttgart, Pfaffenwaldring 9, 70569 Stuttgart, Germany. mauch@ito.uni-stuttgart.de
Optics Express
|October 6, 2012
Summary
Confocal sensors excel with rough surfaces, but artifacts arise on curved ones. This study extends the signal model to explain these measurement errors on non-planar geometries.
Area of Science:
- Optical metrology
- Surface characterization
- Sensor technology
Background:
- Confocal sensors are widely used for surface measurements due to their robustness, particularly on rough surfaces.
- The established signal model is derived assuming planar surfaces or point-like objects, limiting its applicability.
- Measurement artifacts have been observed when using confocal sensors on curved surfaces.
Purpose of the Study:
- To investigate and explain the origin of artifacts in confocal point sensor measurements of curved surfaces.
- To extend the conventional confocal sensor signal model to account for curved geometries.
- To provide a qualitative explanation for observed measurement deviations.
Main Methods:
- Experimental measurement of a surface standard using a confocal point sensor.
- Analysis of measurement data to identify and characterize artifacts on curved sections.
- Development and application of an extended signal model to interpret the experimental results.
Main Results:
- Experimental data clearly demonstrates the emergence of significant artifacts during confocal point sensor measurements of curved surfaces.
- The extended signal model successfully explains the qualitative behavior of these artifacts.
- Deviations from the conventional model are directly linked to the surface curvature.
Conclusions:
- The conventional confocal sensor signal model is insufficient for accurately measuring curved surfaces.
- An extended signal model provides a framework for understanding and potentially mitigating artifacts on curved geometries.
- This work advances the understanding of confocal sensor limitations and opens avenues for improved measurement accuracy on complex surfaces.
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