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Related Experiment Video

Updated: May 18, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Lens-less surface second harmonic imaging.

Krystal L Sly¹, Trang T Nguyen, John C Conboy

¹Department of Chemistry, University of Utah, 315 South 1400 East, Rm 2020, Salt Lake City, Ut 84112, USA.

|October 6, 2012

Summary

This summary is machine-generated.

Lens-less surface second harmonic generation imaging (SSHGI) enables direct imaging of molecules without lenses. This technique offers high resolution and efficiency, paving the way for advanced biosensors and medical diagnostics.

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Area of Science:

Optics and Photonics
Biophysics
Materials Science

Background:

Second Harmonic Generation Imaging (SHGI) is a nonlinear optical microscopy technique.
Conventional SHGI often requires complex lens systems for imaging.
Developing lens-less imaging methods can simplify instrumentation and improve efficiency.

Purpose of the Study:

To demonstrate the capability of lens-less surface second harmonic generation imaging (SSHGI).
To image a specific SHG-active molecule, (S)-(+)-1,1'-bi-2-naphthol (SBN), using SSHGI.
To evaluate the resolution and performance of lens-less SSHGI at various distances.

Main Methods:

Utilized lens-less surface second harmonic generation imaging (SSHGI).
Imaged (S)-(+)-1,1'-bi-2-naphthol (SBN) incorporated into a patterned lipid bilayer.
Employed the 1951 United States Air Force resolution test target for resolution assessment.

Main Results:

Demonstrated direct imaging of SBN without a lens system due to the coherent plane-wave nature of SHG.
Achieved resolution of line-widths as small as 223 μm at 7.6 cm and 397 μm at 30 cm.
Showcased simplified detection, increased photon collection efficiency, and expanded field-of-view.

Conclusions:

Lens-less SSHGI is a viable method for direct molecular imaging.
The technique offers significant advantages in simplicity, efficiency, and field-of-view.
Lens-less SSHGI holds potential for applications in biosensors and medical diagnostics.