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Related Experiment Video

Updated: Jun 24, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

Comprehensive resolution and classification of the Epstein Barr virus transcriptome.

Truong D Nguyen¹, Jia Wang¹, Meggie T Lam¹

¹Department of Pathology & Laboratory Medicine, Tulane University School of Medicine, New Orleans, LA, USA.

Nature Communications

|July 10, 2025

Summary

This study reveals extensive Epstein-Barr virus (EBV) transcriptome diversity using long-read sequencing. Researchers identified numerous transcript isoforms and regulatory mechanisms governing EBV lytic gene expression.

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Last Updated: Jun 24, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

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Published on: November 8, 2011

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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

Area of Science:

Virology
Molecular Biology
Genomics

Background:

Epstein-Barr virus (EBV) genomes contain compact gene and regulatory element repertoires.
Understanding EBV's complex transcriptome is crucial for deciphering its lytic gene expression and regulation.

Purpose of the Study:

To conduct a comprehensive transcriptome analysis of EBV using long-read sequencing.
To identify and categorize viral transcript isoforms based on their dependence on viral DNA replication.
To elucidate the regulatory mechanisms governing EBV lytic gene expression.

Main Methods:

Long-read sequencing for transcriptome analysis.
Identification and categorization of transcript isoforms.
Genetic and chromatin interaction studies.
Analysis of viral DNA replication dependence.

Main Results:

Identified 1453 transcript isoforms for Epstein-Barr virus (EBV).
Resolved major isoforms for almost all lytic reading frames.
Discovered biphasic promoters and enhancer function for the lytic origin of replication (OriLyt).
Observed significant viral read-through transcription impacting gene expression and lncRNA production.

Conclusions:

The study reveals extensive viral transcriptome diversity in EBV.
Major isoforms for nearly all lytic ORFs were resolved.
Alternative regulatory modes driving temporal EBV lytic gene expression were identified.