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Updated: May 18, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Phase shifting technique for extended inline holographic microscopy with a pinhole array.

Christian Graulig¹, Mario Kanka, Rainer Riesenberg

¹Institute of Photonic Technology, Albert-Einstein-Str 9, 07747 Jena, Germany.

|October 6, 2012

Summary

This summary is machine-generated.

This study presents a new digital inline holographic microscopy technique using a 2D pinhole array for improved sample imaging. The method successfully reconstructed amplitude and phase images of dense blood samples.

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Area of Science:

Optics and Imaging
Microscopy
Biomedical Engineering

Background:

Digital inline holographic microscopy (DIHM) offers lensless imaging capabilities.
Traditional DIHM setups face limitations in sample size and density due to single-point illumination.
Generating multiple reference waves is crucial for overcoming these limitations.

Purpose of the Study:

To develop an advanced DIHM technique for imaging dense and large samples.
To enhance sample illumination by utilizing a 2D pinhole array.
To achieve high-resolution amplitude and phase imaging of biological samples.

Main Methods:

Implemented a 2D pinhole array for generating multiple, phase-shifted reference waves.
Utilized phase shifting with stable phase differences and low phase error (rms of 0.027 rad).
Applied the technique to illuminate an undiluted, dense blood smear sample.

Main Results:

Successfully reconstructed both amplitude and phase images of the blood sample.
Demonstrated the capability of the 2D pinhole array to handle dense sample conditions.
Validated the effectiveness of phase-shifting for improved holographic reconstruction.

Conclusions:

The developed DIHM technique effectively overcomes sample size and density restrictions.
Phase-shifting in a 2D pinhole array illumination enables high-quality lensless imaging of challenging samples.
This method holds potential for advanced biomedical imaging applications.