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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Related Experiment Video

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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Depth-filtered digital holography.

Nektarios Koukourakis1, Volker Jaedicke, Adamou Adinda-Ougba

  • 1Photonics and Terahertz-Technology, Ruhr-University Bochum, Universitätsstr 150,44801 Bochum, Germany. Nektarios.Koukourakis@rub.de

Optics Express
|October 6, 2012
PubMed
Summary
This summary is machine-generated.

Depth-filtered digital holography (DFDH) enables quantitative phase imaging of buried layers in multilayer samples. This novel method reconstructs depth profiles by analyzing wavelength-dependent holograms, revealing internal structures with high precision.

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Area of Science:

  • Optics and Photonics
  • Materials Science
  • Imaging Technologies

Background:

  • Quantitative phase imaging is crucial for analyzing transparent and multilayered materials.
  • Existing methods often struggle with depth resolution for buried structures.
  • Digital holography offers potential but requires advanced techniques for depth profiling.

Purpose of the Study:

  • To introduce and validate depth-filtered digital holography (DFDH) for tomographic phase imaging.
  • To enable non-destructive, quantitative analysis of buried layers within multilayered samples.
  • To develop a method for isolating and imaging specific depths of interest (DOI).

Main Methods:

  • Acquisition of multiple holograms at varying wavelengths.
  • Pixel-wise intensity analysis over wavelength coupled with inverse Fourier transforms for depth profiling.
  • Application of windowed Fourier transforms to select a specific depth-of-interest (DOI).
  • Utilizing the angular spectrum method for spatial filtering and hologram reconstruction.

Main Results:

  • Demonstrated proof-of-principle for DFDH in experimental settings.
  • Successful quantitative tomographic phase imaging of buried layers.
  • Generation of depth-resolved phase information from multilayered samples.
  • Filtered interference patterns containing information from a selected DOI.

Conclusions:

  • DFDH is a viable technique for quantitative imaging of buried layers.
  • The method provides depth-resolved phase information in multilayered samples.
  • DFDH offers a new approach for non-destructive internal structure analysis.