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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

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Published on: June 28, 2024

Switchable nanodumbbell probes for analyte detection.

Phyllis F Xu¹, Albert M Hung, Hyunwoo Noh

¹Department of Nanoengineering and Materials Science Engineering Program, University of California, San Diego, La Jolla, CA 92093-0448, USA.

Small (Weinheim an Der Bergstrasse, Germany)

|October 9, 2012

Summary

This summary is machine-generated.

Gold nanoparticle dimers linked by DNA change their shape when detecting adenosine triphosphate (ATP). This DNA-based sensing mechanism causes a significant decrease in particle distance, enabling analyte detection.

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Area of Science:

Nanotechnology
Biochemistry
Materials Science

Background:

Gold nanoparticles (AuNPs) are widely used in biosensing applications.
DNA nanotechnology offers precise control over nanoparticle assembly and spacing.
Analyte-induced conformational changes in DNA can be harnessed for sensing.

Purpose of the Study:

To develop a novel sensing platform using gold nanoparticle dimers.
To investigate the effect of adenosine triphosphate (ATP) on DNA-linked AuNP dimers.
To demonstrate analyte-induced structural changes for detection.

Main Methods:

Synthesis of nanodumbbell gold nanoparticle dimers.
Functionalization of AuNPs with DNA linkers.
Characterization of interparticle distance using spectroscopy or microscopy.
Exposure to target analyte (ATP) and observation of structural response.

Main Results:

AuNP dimers were successfully synthesized and linked with DNA.
In the presence of ATP, DNA linkers formed stable hairpin structures.
This hairpin formation led to a significant decrease in the interparticle distance of the AuNP dimers.

Conclusions:

Nanodumbbell AuNP dimers with DNA linkers can act as sensitive biosensors.
The analyte-induced DNA hairpin formation is a viable mechanism for detecting ATP.
This approach offers a promising route for developing distance-based nanoparticle sensors.