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Updated: May 17, 2026
Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies
Published on: January 19, 2022
Bioassay evaluation on the efficacy of α-cypermethrin impregnated into long lasting insecticide treated nets using
Vatandoost Hassan1, Mamivand Poor Hossein, Shayeghi Mansoreh
1Department of Medical Entomology & Vector Control, School of Public Health & National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran. hvatandoost1@yahoo.com
Objective:
To evaluate the bioefficacy of α-cypermethrin impregnated into long lasting insecticide treated nets (LLITNs-INTERCEPTOR®) against main malaria vector, Anopheles stephensi (An. stephensi).
Methods:
The effectiveness of bed net impregnated with α-cypermethrin (INTERCEPTOR®) with washing was evaluated. The washing procedure and bioassay tests were carried out according to the WHO-recommended methods. Malaria vector, An. stephensi was exposed to impregnated bed net for three minutes and then mortality measured after 24 h recovery period. Knockdown was also measured according to the logarithmic times.
Results:
Result of cone bioassay method showed that bioefficacy of α-cypermethrin decreased from 100% in unwashed to 15% in 20 washes. KT(50) was measure as one minute in one wash and increased to 40 min in 20 washes.
Discussion:
Findings of this study provide guideline for malaria vector control authorities and people using pyrethroid-impregnated bed nets.
