Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bone Marrow Sampling and Transplants

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Consensus document on the implications of standardization of BCG supply in the management of patients with non-muscle-invasive bladder cancer.

Actas urologicas espanolas·2024

Same author

Hormone sensitive protocol for metastatic prostate cancer, an update.

Actas urologicas espanolas·2022

Same author

Non-metastatic castration-resistant prostate cancer: management recommendations.

Actas urologicas espanolas·2022

Same author

Mathematical model optimized for prediction and health care planning for COVID-19.

Medicina intensiva·2022

Same author

Role of normothermic perfusion with ECMO in donation after controlled cardiac death in Spain.

Medicina intensiva·2021

Same author

Mathematical model optimized for prediction and health care planning for COVID-19.

Medicina intensiva·2021

Search research articles

Related Experiment Video

Updated: May 17, 2026

A High-Fidelity Porcine Model of Orthotopic Heart Transplantation Following Donation after Circulatory Death

A High-Fidelity Porcine Model of Orthotopic Heart Transplantation Following Donation after Circulatory Death

Published on: June 6, 2025

Alpha code. The start of a new non-heart beating donor program.

J M Pérez-Villares¹, R Lara-Rosales, F Pino-Sánchez

¹Coordinación de Trasplantes y Unidad de Medicina Intensiva, Servicio de Cuidados Críticos y Urgencias, Hospital Universitario Virgen de las Nieves, Granada, España. josem.perez.villares.sspa@juntadeandalucia.es

Medicina Intensiva

|October 10, 2012

Summary

This summary is machine-generated.

Non-heart beating donor (NHBD) programs are viable in smaller cities. NHBDs contributed significantly to organ donation and transplantation in Granada, supporting program development.

More Related Videos

Pre-clinical Model of Cardiac Donation after Circulatory Death

Pre-clinical Model of Cardiac Donation after Circulatory Death

Published on: August 2, 2019

Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death

Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death

Published on: August 15, 2022

Related Experiment Videos

Last Updated: May 17, 2026

A High-Fidelity Porcine Model of Orthotopic Heart Transplantation Following Donation after Circulatory Death

A High-Fidelity Porcine Model of Orthotopic Heart Transplantation Following Donation after Circulatory Death

Published on: June 6, 2025

Pre-clinical Model of Cardiac Donation after Circulatory Death

Pre-clinical Model of Cardiac Donation after Circulatory Death

Published on: August 2, 2019

Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death

Lung Rapid Recovery Procurement Combined with Abdominal Normothermic Regional Perfusion in Controlled Donation after Circulatory Death

Published on: August 15, 2022

Area of Science:

Organ transplantation
Nephrology
Public health

Background:

Organ donation programs are crucial for saving lives.
Establishing non-heart beating donor (NHBD) programs in smaller cities requires careful evaluation.
Brain dead donors (BDD) are the traditional source, but NHBDs offer additional potential.

Purpose of the Study:

To evaluate the feasibility and preliminary outcomes of a non-heart beating donor (NHBD) program in a city with a population under 500,000.
To compare NHBD program results with established brain dead donor (BDD) programs within the same region.

Main Methods:

A prospective observational study was conducted in Granada, Spain, from 2010 to 2011.
Data collected included donor characteristics, organ non-validity reasons, preservation techniques, and recipient outcomes.
Comparison was made between NHBD and BDD data, including kidney transplants and overall organ donation rates.

Main Results:

The NHBD program identified 20 real donors, resulting in 21 kidney and 2 liver transplants.
Reasons for organ non-validity were documented.
Recipient outcomes showed a mean of 1.4 post-transplant hemodialysis sessions and a mean hospital stay of 25 days.

Conclusions:

Preliminary results indicate that NHBD programs can be successfully implemented in cities with populations under 500,000.
In 2011, NHBDs constituted over 20% of kidney transplants and global organ donations in the Granada province.
The study supports the expansion of NHBD programs to increase organ availability.