Isolation of cerebellar granule cells from neonatal rats

Cerebellar Granule Cells in Neurotoxicology (Jan Oberdoerster, Aventis Corporation, Research Triangle Park, North Carolina). Cultured neurons allow the researcher to investigate mechanisms of toxicity on a relatively uniform population of cells. Primary cultures of cerebellar granule cells are post-mitotic neurons that are readily isolated and may be used for experimental procedures including electrophysiology, neuronal maturation, and various biochemical and molecular analyses.