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Updated: May 17, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Measurement of glutathione and glutathione disulfide
1Arizona Cancer Center, Tucson, Arizona, USA.
Abstract:
Measurements of glutathione should include quantification of both the reduced and oxidized forms. HPLC-based assays for glutathione and other cellular thiols and disulfides utilize a variety of detection methods, including ultraviolet, fluorescence, and electrochemical methods. This unit provides rapid, sensitive, highly versatile and reproducible HPLC methods for separating and quantifying sulfur-containing amino acids and related derivatives from a variety of biological samples.
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