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Measurement of δ-aminolevulinate dehydratase activity
1Hokkaido University School of Medicine, Sapporo, Japan.
Current Protocols in Toxicology
|October 10, 2012
Abstract:
Vertebrate ALAD is a cytosolic enzyme that catalyzes the second step of porphyrin formation, and it is expressed abundantly in liver and erythroid tissues as well as in other tissues. This unit describes a colorimetric assay for measuring ALAD activity in mammalian blood or tissues and the extent of reactivation of the enzyme in lead-inhibited samples.
