Related Experiment Video
Updated: May 17, 2026
09:43
Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Detection of heme oxygenase activity by measurement of CO
1Stanford University Medical Center, Stanford, California, USA.
Current Protocols in Toxicology
|October 10, 2012
Abstract:
Heme oxygenase (HO) is the first and rate-limiting step in degradation of heme, and in the presence of NADPH-cytochrome P-450 reductase it produces equimolar amounts of biliverdin and CO. CO produced in a closed system can be quantified as described in this unit by gas chromatography as a measure of HO activity in tissue slices, tissue homogenates, and tissue fractions.
