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Updated: May 17, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Histochemical analysis of heme degradation enzymes
1ArQule, Inc., Waltham, Massachusetts, USA.
Abstract:
Histochemical analysis can be used to study the cellular localization, expression, and regulation of enzymes responsible for catabolism of the heme molecule. This unit describes protocols for assessing the expression and regulation of specific HO-1 and HO-2 mRNAs by in situ hybridization using digoxigenin-tagged probes. Immunohistochemistry is used to visualize specific cellular sites of HO-1 and HO-2 protein expression.
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