Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Potential protection of skin by acute UVA irradiation--from cellular to animal models.

Progress in biophysics and molecular biology·2006

Same author

Phosphatidylinositol 3-kinase/Akt pathway mediates heme oxygenase-1 regulation by lipopolysaccharide.

Cellular and molecular biology (Noisy-le-Grand, France)·2005

Same author

Solar ultraviolet A radiation: an oxidizing skin carcinogen that activates heme oxygenase-1.

Antioxidants & redox signaling·2004

Same author

Tea flavonoids and cardiovascular health.

QJM : monthly journal of the Association of Physicians·2001

Same author

The heme synthesis and degradation pathways: role in oxidant sensitivity. Heme oxygenase has both pro- and antioxidant properties.

Free radical biology & medicine·2001

Same author

Epicatechin and its in vivo metabolite, 3'-O-methyl epicatechin, protect human fibroblasts from oxidative-stress-induced cell death involving caspase-3 activation.

The Biochemical journal·2001

Search research articles

Related Experiment Video

Updated: May 17, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

An HPLC method to detect heme oxygenase activity.

S W Ryter¹, R M Tyrrell

¹Southern Illinois University School of Medicine, Springfield, Illinois, USA.

Current Protocols in Toxicology

|October 10, 2012

Summary

This study introduces a new assay to measure heme oxygenase activity by quantifying bilirubin products. The method simplifies heme degradation analysis by directly measuring biliverdin and bilirubin without needing extra enzymes.

Area of Science:

Biochemistry
Enzymology

Background:

Heme oxygenase (HO) is a key enzyme in heme catabolism.
Accurate measurement of HO activity is crucial for understanding cellular processes and disease.
Existing assays may require specific cofactors or have limitations.

Purpose of the Study:

To develop and validate a novel method for quantifying heme oxygenase enzymatic activity.
To establish an assay that directly measures heme degradation products.

Main Methods:

Quantification of biliverdin-IX alpha (BV) and bilirubin-IX alpha (BR) using reversed-phase High-Performance Liquid Chromatography (RP-HPLC).
Detection of BV and BR via visible absorbance spectroscopy.
Calculation of heme oxygenase activity based on the direct measurement of generated metabolites.

More Related Videos

Biochemical Measurement of Neonatal Hypoxia

Biochemical Measurement of Neonatal Hypoxia

Published on: August 24, 2011

Related Experiment Videos

Last Updated: May 17, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

Biochemical Measurement of Neonatal Hypoxia

Biochemical Measurement of Neonatal Hypoxia

Published on: August 24, 2011

Main Results:

The assay accurately determines heme oxygenase activity by measuring BV and BR formation.
Reversed-phase HPLC coupled with visible absorbance spectroscopy provides reliable metabolite detection.
The method eliminates the need for biliverdin reductase supplementation, simplifying the procedure.

Conclusions:

This novel assay provides a robust and simplified method for assessing heme oxygenase enzymatic activity.
The direct quantification of heme degradation products offers an improved approach for biochemical research.