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Updated: May 17, 2026
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Experimental Approaches for Biochemical Analysis of Glial Fibrillary Acidic Protein and Its Disease-associated Variants
Published on: November 28, 2025
Measurement of glial fibrillary acidic protein
1Center for Disease Control and Prevention NIOSH, Morgantown, WV, USA.
Current Protocols in Toxicology
|October 10, 2012
Abstract:
Toxicant-induced injury of the adult or developing central nervous system results in hypertrophy of astrocytes at the site of injury. The hallmark of this response is enhanced expression of the major intermediate filament protein of astrocytes, glial fibrillary acid protein (GFAP). A protocol is provided for assaying GFAP levels in detergent homogenates of brain tissue using a sandwich ELISA.
