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Updated: May 17, 2026

Quantification of Coenzyme A in Cells and Tissues
Published on: September 27, 2019
Coenzyme A and coenzyme A-glutathione mixed disulfide measurements by HPLC
Lynette K Rogers1, Charles V Smith
1Children's Research Institute, The Ohio State University, Columbus, OH, USA.
Abstract:
This unit describes a method for measuring coenzyme A and coenzyme A-glutathione mixed disulfide in tissue homogenates obtained from tissues frozen in liquid nitrogen, homogenized in the presence of N-ethylmaleimide, and then acidified. to avoid the oxidation, reduction, and thiol exchange reactions that can occur during tissue processing and isolation of subcellular fractions. This method may provide a way to assess changes in the oxidative state of mitochondria in intact tissues.
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