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In situ localization of nonenzymatic peroxidase-like activity of tissue-bound transition metals

Lawrence M Sayre1, Peggy L R Harris, George Perry

  • 1Case Western Reserve University, Cleveland, OH, USA.

Current Protocols in Toxicology
|October 10, 2012
PubMed

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Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...