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Related Experiment Video

Updated: May 17, 2026

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Published on: February 9, 2020

Local lymph node assays.

Rebecca J Dearman¹, Ian Kimber

¹Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, United Kingdom.

Current Protocols in Toxicology

|October 10, 2012

Summary

This summary is machine-generated.

The murine local lymph node assay (LLNA) identifies skin sensitizers and allergic contact dermatitis potential. This method offers a reliable alternative to guinea pig tests for hazard assessment and risk evaluation.

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Area of Science:

Toxicology
Immunology

Background:

Skin sensitization and allergic contact dermatitis are significant health concerns.
Traditional methods using guinea pigs have limitations.
The murine local lymph node assay (LLNA) emerged as an alternative approach.

Purpose of the Study:

To present and validate the murine local lymph node assay (LLNA) for identifying skin sensitizers.
To offer a robust and reliable alternative to traditional animal testing methods.
To enable quantitative risk assessment through relative potency measurements.

Main Methods:

The LLNA measures proliferative responses in draining lymph nodes following chemical exposure.
Radiolabeled thymidine incorporation is used to quantify cellular proliferation.
Two protocols are detailed: one with pooled lymph nodes and another with individual lymph node analysis for statistical power.

Main Results:

The LLNA effectively identifies chemicals with skin sensitization potential.
The assay provides a reliable method for hazard assessment.
Data generated can be utilized for relative potency determination in risk assessments.

Conclusions:

The LLNA is a validated and reliable method for assessing skin sensitization hazards.
It serves as a viable alternative to older guinea pig tests.
The assay supports both hazard identification and risk assessment for contact allergens.