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Related Experiment Video

Updated: May 17, 2026

Murine Model of Allergen Induced Asthma
08:05

Murine Model of Allergen Induced Asthma

Published on: May 14, 2012

Murine asthma models.

Jean F Regal1

  • 1School of Medicine, University of Minnesota, Duluth, MN, USA.

Current Protocols in Toxicology
|October 10, 2012
PubMed
Summary
This summary is machine-generated.

This study details an ovalbumin-induced asthma model in mice to identify asthma-exacerbating exposures. The model effectively replicates key asthma symptoms like airway hyperresponsiveness and eosinophil infiltration.

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Area of Science:

  • Toxicology
  • Immunology
  • Respiratory Medicine

Background:

  • Immunotoxicity can manifest as hypersensitivity, potentially leading to lung conditions like asthma.
  • Asthma is characterized by reversible airway obstruction, eosinophil infiltration, and airway hyperresponsiveness.
  • Toxicological research focuses on identifying respiratory allergens and exposures that worsen existing asthma.

Purpose of the Study:

  • To describe a protocol for an ovalbumin-induced asthma model in mice.
  • To evaluate this model for its ability to identify asthma-exacerbating exposures.
  • To establish methods for assessing key asthma-related endpoints in mice.

Main Methods:

  • Mice were sensitized and challenged with ovalbumin to induce asthma.
  • Airway hyperresponsiveness was measured using whole-body plethysmography following methacholine challenge.
  • Cellular infiltration in the lungs was assessed via bronchoalveolar lavage and lung tissue analysis (eosinophil peroxidase, myeloperoxidase).

Main Results:

  • The ovalbumin-induced model successfully generated asthma symptoms, including airway hyperresponsiveness and eosinophil infiltration.
  • The methods described allow for quantitative assessment of immune cell presence in the lungs.
  • Further analyses of bronchoalveolar lavage fluid and lung tissue can provide detailed molecular insights.

Conclusions:

  • An ovalbumin-induced mouse model is effective for studying asthma exacerbation.
  • This model facilitates the identification of environmental factors that worsen asthma.
  • The described protocol provides a comprehensive approach to evaluating asthma pathophysiology in a preclinical setting.