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Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
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Published on: March 23, 2015

Analysis of mitochondrial dysfunction during cell death.

Vladimir Gogvadze1, Sten Orrenius, Boris Zhivotovsky

  • 1Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Current Protocols in Toxicology
|October 10, 2012
PubMed
Summary
This summary is machine-generated.

This study details protocols for assessing mitochondrial function during apoptosis, a key cell death pathway. Mitochondria play a crucial role in apoptosis, and these methods help analyze their involvement.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Apoptosis, or programmed cell death, is a fundamental biological process.
  • Mitochondria are central to the apoptotic pathway, responding to various cytotoxic stimuli.
  • Identifying common steps in apoptosis is crucial for understanding cell death.

Purpose of the Study:

  • To provide a comprehensive set of protocols for evaluating mitochondrial functions during apoptosis.
  • To facilitate the investigation of mitochondria's role in diverse apoptotic responses.
  • To standardize the assessment of mitochondrial involvement in cell death.

Main Methods:

  • Collection and analysis of proteins released from mitochondria.
  • Detection of the mitochondrial permeability transition.
  • Measurement of mitochondrial membrane potential.
  • Preparation of mitochondria from various tissue sources.

Main Results:

  • Established protocols enable detailed analysis of mitochondrial apoptotic functions.
  • Methods allow for the characterization of mitochondrial changes during cell death.
  • The protocols are applicable across different tissue types.

Conclusions:

  • Mitochondrial function assessment is vital for understanding apoptosis.
  • These protocols offer a robust toolkit for researchers studying cell death.
  • Standardized methods improve the reliability of apoptosis research.