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Updated: May 17, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
Analysis of mitochondrial dysfunction during cell death
Vladimir Gogvadze1, Sten Orrenius, Boris Zhivotovsky
1Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Abstract:
Attempts to identify a common underlying step in the apoptotic program in response to various cytotoxic stimuli have focused on the role of mitochondria in this form of cell death. This unit contains a family of protocols that can be used to assess mitochondrial functions during apoptotic responses. Protocols are included for the collection and analysis of released proteins, for detection of the mitochondrial permeability transition, for measurement of mitochondrial membrane potential, and for preparation of mitochondria from different tissue sources.
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