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Jagged-1-mediated activation of notch signalling induces adipogenesis of adipose-derived stem cells

K Ba1, X Yang, L Wu

  • 1State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.

Cell Proliferation
|October 11, 2012
PubMed
Abstract

Insights

Activation of Notch signaling, using Jagged1, promotes adipogenesis in mouse adipose-derived stem cells (mASCs). This process involves increased expression of downstream Notch genes and the key adipogenic transcription factor PPAR-γ.

Area of Science:

  • Stem cell biology
  • Cell signaling pathways
  • Adipogenesis research

Background:

  • Notch signaling is crucial for various cellular processes, including proliferation, migration, differentiation, and cell death.
  • Understanding its role in adipogenesis is vital for stem cell research.

Purpose of the Study:

  • To investigate the effects of Notch signaling activation on the adipogenesis of mouse adipose-derived stem cells (mASCs).

Main Methods:

  • mASCs were treated with Jagged1 to activate Notch signaling prior to adipogenic induction.
  • Lipid accumulation was assessed using Oil Red-O staining.
  • Gene and protein expression of Notch downstream targets (Notch-1, -2, Hes-1, Hey-1) and PPAR-γ were analyzed via real-time PCR and immunofluorescence.

Main Results:

  • Jagged1 treatment significantly promoted adipogenic differentiation of mASCs.
  • Expression levels of Notch downstream genes and PPAR-γ were elevated in Jagged1-treated cells.
  • Enhanced expression of Hes-1 and PPAR-γ proteins was confirmed in pre-treated mASCs.

Conclusions:

  • Activation of Notch signaling stimulates adipogenesis in mASCs.
  • This stimulation is mediated by the promotion of PPAR-γ expression within the adipogenic medium.

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