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Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia
Published on: November 10, 2023
Soluble mediators released by acute myeloid leukemia cells increase capillary-like networks
Kimberley J Hatfield1, Lasse Evensen, Håkon Reikvam
1Section for Hematology, Department of Medicine, Haukeland University Hospital, and Department of Hematology, University of Bergen, Bergen, Norway. mmakh@med.uib.no
European Journal of Haematology
|October 11, 2012
Summary
Leukemic cells in acute myeloid leukemia (AML) can stimulate blood vessel formation. Specific cytokine profiles in AML patients drive this increased angiogenesis, impacting disease progression.
Area of Science:
- Hematology
- Oncology
- Cell Biology
Background:
- Bone marrow angiogenesis is elevated in hematological malignancies like acute myeloid leukemia (AML).
- Understanding the role of leukemic cells in promoting angiogenesis is crucial for AML research.
Purpose of the Study:
- To investigate the impact of acute myeloid leukemia (AML)-conditioned medium on endothelial cell network formation.
- To identify specific cytokines released by AML cells that influence angiogenesis.
Main Methods:
- Co-culture assay using endothelial cells and vascular smooth muscle cells (vSMC).
- Analysis of cytokine profiles from primary AML cells of 44 patients.
- Hierarchical cluster analysis of cytokine data to identify patient subsets.
Main Results:
- AML-conditioned medium from a subset of patients significantly stimulated capillary-like network formation.
- High constitutive release of pro-angiogenic chemokines correlated with enhanced network formation.
- Acute lymphoblastic leukemia cells did not show similar effects; activated T cells and monocytes had minimal impact.
Conclusions:
- Cytokines released by AML cells can induce endothelial cell organization into vessel-like structures.
- AML patients can be categorized into subsets based on their angiogenic potential.
- Targeting AML-driven angiogenesis may offer new therapeutic strategies.
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