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Published on: August 13, 2013
[Inhibitory effect of the recombinant adenovirus pre-miR-10a on K562 cell proliferation]
Jie-yu Qi1, Kun Tao, Can-wei Wang
1Department of Immunology, Center for Molecular Medicine and Cancer Research, Chongqing, China.
Aim:
To investigate the changes of proliferation and apoptosis in K562 cells over-expressing miR-10a.
Methods:
K562 cells were infected with pAd-pre-miR-10a virus, and then the levels of miR-10a and bcr/abl in transfected K562 cells was detected using RT-PCR and the level of BCR/ABL using Western blotting. The methylthiazolyl tetrazolium (MTT) assay and flow cytometry (FCM) were used to monitor the changes of proliferation and apoptosis of K562 cells after transfection.
Results:
Compared with the control groups, K562 cells transfected with pAd-pre-miR-10a presented the significantly increased level of miR-10a, the significantly decreased expressions of bcr/abl fusion gene and BCR/ABL fusion protein, the inhibited proliferation ability and the promoted apoptosis (P<0.05).
Conclusion:
The recombinant adenovirus of pAd-pre-miR-10a can inhibit the proliferation of K562 cells and promote cell apoptosis significantly.
