HPLC method development and validation of chromafenozide in paddy

Area of Science:

• Agricultural Chemistry
• Analytical Chemistry
• Environmental Science

Background:

• No established analytical methods existed for chromafenozide determination in paddy.
• Understanding chromafenozide residue behavior is crucial for agricultural safety and environmental monitoring.

Purpose of the Study:

• To develop and validate a simple, efficient High-Performance Liquid Chromatography with Ultraviolet detection (HPLC-UV) method for chromafenozide analysis.
• To investigate the residue analysis, dissipation kinetics, and final residue levels of chromafenozide in paddy and associated soil after field application.

Main Methods:

• Extraction and purification of chromafenozide residues from paddy (straw, grain) and soil samples.
• Analysis using High-Performance Liquid Chromatography with a Photodiode Array (PDA) detector on a Phenomenex Luna RP C(18) column.
• Method validation including accuracy, precision (repeatability and reproducibility), and limit of detection.

Main Results:

• The developed HPLC-UV method demonstrated high accuracy (94.67-96.90%) and acceptable precision (repeatability and reproducibility) across different matrices.
• The minimum detectable concentration was 0.01 mg kg(-1).
• Chromafenozide exhibited rapid degradation, with half-lives of approximately 4.4 days in paddy plants and 2.9 days in soil at double the recommended dose.

Conclusions:

• The developed HPLC-UV method is suitable for the accurate and efficient determination of chromafenozide residues in paddy.
• The study provides valuable data on chromafenozide dissipation, indicating its relatively short persistence in the agricultural environment.