Related Experiment Video
Updated: May 17, 2026
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
The effect of long zona dissection using ICSI pipettes for mechanical assisted hatching in vitrified-thawed
Sheng-Ta Sun1, Jong-Ryeol Choi, Jung-Bin Son
1Department of Obstetrics and Gynecology, Pusan National University, School of Medicine, Gu-Duk-Ro 305, Su-Gu, Busan, Republic of Korea.
Objective:
To investigate the effect of long zona dissection (LZD) compared with partial zona dissection (PZD) using ICSI pipettes for mechanical assisted hatching (AH) in vitrified-thawed blastocyst transfers.
Design:
Prospective study.
Setting:
University IVF clinic.
Patient(S):
A total of 120 women ≦ 38 years old undergone vitrified-thawed blastocyst transfers with LZD or PZD.
Intervention(S):
The culture of all pronucleate embryos to the blastocyst stage and the selection of blastocysts ≧ grade 3BB (Gardner and Schoolcraft score), followed by vitrified-thawed blastocyst transfers with LZD (n = 60) or with PZD (n = 60)
Main Outcome Measure(S):
Complete hatching rates, implantation rates, pregnancy rates.
Result(S):
At 5 h after thawing, complete hatching rates of blastocysts were significantly higher in LZD group compared with PZD group, 52.4 % vs. 31.8 % (P = 0.001). Implantation and clinical pregnancy rates were significantly higher in LZD group compared with PZD group, 40.9 % vs. 25.7 % and 63.0 % vs. 40.0 %, respectively (P = 0.010, P = 0.011).
Conclusion(S):
LZD using ICSI pipettes for mechanical AH improves significantly complete hatching, implantation and pregnancy rates in vitrified-thawed blastocyst transfers.
