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Related Experiment Video

Updated: May 17, 2026

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
08:43

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Published on: April 3, 2018

Subcutaneous tissue responses to three endodontic irrigants: a comparative study.

Kazem Ashofteh Yazdi1, Mohammad Sabeti, Pooriya Motahhary

  • 1Dental Research Center, Department of Endodontics, Dental School, Tehran University of Medical Sciences, Tehran, Iran.

Iranian Endodontic Journal
|October 12, 2012
PubMed
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The mixture of a tetracycline isomer, an acid, and a detergent (MTAD) showed similar subcutaneous tissue toxicity to 2.6% sodium hypochlorite (NaOCl) and 17% ethylenediaminetetraacetic acid (EDTA) in rats.

Area of Science:

  • Biomaterials Science
  • Dental Materials
  • Histopathology

Background:

  • Evaluating the biocompatibility of endodontic irrigants is crucial for clinical safety.
  • MTAD, 17% EDTA, and 2.6% NaOCl are commonly used dental irrigants with varying proposed mechanisms and potential tissue interactions.

Purpose of the Study:

  • To histologically compare the subcutaneous tissue responses to MTAD, 17% EDTA, and 2.6% NaOCl.
  • To assess the inflammatory potential and toxicity of these irrigants at different time points post-injection.

Main Methods:

  • Subcutaneous injections of MTAD, 17% EDTA, and 2.6% NaOCl were administered to Wistar albino rats.
  • Rats were sacrificed at 2 hours, 2 days, and 2 weeks, with histological assessment of inflammation severity.
  • Statistical analysis included Kruskal-Wallis and Friedman tests.
Keywords:
EDTAInflammationMTADSodium hypochloriteSubcutaneous TissueToxicity

Related Experiment Videos

Last Updated: May 17, 2026

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
08:43

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Published on: April 3, 2018

Main Results:

  • Significant differences in inflammatory reactions were observed among the irrigants over time (P<0.05).
  • MTAD and 2.6% NaOCl induced comparable levels of inflammation at all time points (P>0.05).
  • MTAD and 17% EDTA showed similar subcutaneous tissue responses at 2-hour and 2-day intervals (P>0.05).

Conclusions:

  • MTAD exhibits comparable subcutaneous tissue toxicity to 2.6% NaOCl under the study conditions.
  • The findings suggest similar biocompatibility profiles for MTAD and NaOCl in this experimental model.