[Determination of 2,4,6-trichloroanisole in wine by head-space solid phase micro-extraction and gas...

To establish a method of combining gas chromatography-mass spectrometry (GC-MS) with head-space solid phase micro-extraction (HS-SPME) for the detection of 2,4,6-trichloroanisole (2,4,6-TCA) in wine.

2,4,6-TCA in wine were extracted by polydimethylsiloxane (PDMS,100 microm) fiber for 15 min under 35 degrees C in a water bath. The fiber was then transferred into the injection port of the GC-MS and separated by a CP-SIL 8CB-MS capillary column (30m x 0.25mm x 0.25 microm), and detected by mass spectrometry using d5-2,4,6-TCA as an internal standard.

The linear range for 2,4,6-TCA was 5 (60ng/L, the detection limit was 0.06ng/L. The recovery of standard addition (5, 10, 20ng/L) was in the range of 79.8% (101.6% with relative standard deviations (RSD, n=5) < or = 7%.

Organic solvents were avoided and the selection was sensitive for the method, which is suitable for the determination of 2,4,6-TCA in wine.