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Hypoxia and oxidative stress markers in pediatric patients undergoing hemodialysis: cross section study

Enas A Hamed1, Taghrid B El-Abaseri, Amany O Mohamed

  • 1Departments of Medical Physiology, Assiut University, Assiut, Egypt. eah3a2010@yahoo.com

BMC Nephrology
|October 16, 2012
PubMed

Insights

Pediatric chronic kidney disease (CKD) patients on hemodialysis (HD) exhibit significant tissue hypoxia and oxidative stress. Hemodialysis (HD) improves hypoxia but reduces antioxidant capacity, highlighting a complex interplay.

Area of Science:

  • Biochemistry
  • Nephrology
  • Pediatrics

Background:

  • Tissue injury from hypoxia and free radicals is prevalent in various diseases.
  • Chronic kidney disease (CKD) and hemodialysis (HD) may impact these processes.
  • This study investigates hypoxia and oxidative stress biomarkers in pediatric CKD patients undergoing HD.

Purpose of the Study:

  • To assess the impact of CKD and HD on hypoxia and oxidative stress markers in children.
  • To compare biomarker levels between CKD patients and healthy controls.
  • To evaluate changes in biomarkers before and after a hemodialysis session.

Main Methods:

  • Cross-sectional study involving 40 pediatric CKD patients on HD and 20 healthy children.
  • Measured plasma hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) via ELISA.
  • Assessed total antioxidant capacity (TAC), total peroxide (TPX), lactate, and pyruvate using colorimetric methods; calculated Oxidative Stress Index (OSI) and Lactate/Pyruvate (L/P) ratio.

Main Results:

  • CKD patients showed higher TPX, OSI, VEGF, and lactate levels compared to controls.
  • HIF-1α was elevated before dialysis compared to controls.
  • Hemodialysis reduced HIF-1α and TAC levels, indicating improved hypoxia but decreased antioxidant capacity post-dialysis.

Conclusions:

  • Pediatric CKD patients experience significant tissue hypoxia and oxidative stress.
  • Hemodialysis partially ameliorates hypoxia but diminishes antioxidant capacity.
  • Biomarker analysis reveals complex effects of CKD and HD on oxidative balance in children.
Abstract

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