Related Experiment Video
Updated: May 17, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Elemental labelling combined with liquid chromatography inductively coupled plasma mass spectrometry for
Daniela Kretschy1, Gunda Koellensperger, Stephan Hann
1University of Natural Resources and Life Sciences, BOKU Vienna, Department of Chemistry, Division of Analytical Chemistry, Muthgasse 18, A-1190 Vienna, Austria.
Abstract:
This article reviews novel quantification concepts where elemental labelling is combined with flow injection inductively coupled plasma mass spectrometry (FI-ICP-MS) or liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS), and employed for quantification of biomolecules such as proteins, peptides and related molecules in challenging sample matrices. In the first sections an overview on general aspects of biomolecule quantification, as well as of labelling will be presented emphasizing the potential, which lies in such methodological approaches. In this context, ICP-MS as detector provides high sensitivity, selectivity and robustness in biological samples and offers the capability for multiplexing and isotope dilution mass spectrometry (IDMS). Fundamental methodology of elemental labelling will be highlighted and analytical, as well as biomedical applications will be presented. A special focus will lie on established applications underlining benefits and bottlenecks of such approaches for the implementation in real life analysis. Key research made in this field will be summarized and a perspective for future developments including sophisticated and innovative applications will given.
More Related Videos
Related Concept Videos
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
High-Performance Liquid Chromatography: Types of Detectors
Mass Spectrometry: Overview
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

