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Related Experiment Video

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Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

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Published on: November 8, 2015

Decrease in circulating autotaxin by oral administration of prednisolone.

Hayakazu Sumida¹, Kazuhiro Nakamura, Keisuke Yanagida

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Tokyo, Japan. sumida-tky@umin.ac.jp

Clinica Chimica Acta; International Journal of Clinical Chemistry

|October 16, 2012

Summary

This summary is machine-generated.

Serum autotaxin (ATX) levels decrease with steroid treatment and recover upon dose reduction, indicating ATX may monitor steroid effectiveness and patient adherence in autoimmune diseases.

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Last Updated: May 17, 2026

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Published on: November 8, 2015

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Area of Science:

Biochemistry
Immunology
Endocrinology

Background:

Autotaxin (ATX) is a lysophospholipase D (lysoPLD) primarily secreted by adipose tissue.
ATX hydrolyzes lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA), influencing immune responses.

Purpose of the Study:

To investigate serum autotaxin (ATX) antigen levels in autoimmune diseases.
To assess the impact of prednisolone (PSL) on ATX levels and expression.

Main Methods:

Serum ATX antigen and lysoPLD activity were measured using automated enzyme immunoassay.
Prednisolone's effect on ATX mRNA expression was evaluated in cultured murine adipose tissue.

Main Results:

Serum ATX antigen levels decreased after initiating PSL treatment.
Decreased ATX levels recovered dose-dependently during PSL tapering without disease exacerbation.
PSL treatment reduced ATX mRNA expression in cultured adipose tissue.

Conclusions:

Serum ATX measurement may be useful for assessing steroid treatment efficacy and compliance.
Findings offer insights into ATX's role in biosynthesis, physiology, pathology, and clinical significance.