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Histological-Based Stainings Using Free-Floating Tissue Sections
Published on: August 25, 2020
Applications of immunohistochemistry.
Jeyapradha Duraiyan1, Rajeshwar Govindarajan, Karunakaran Kaliyappan
1Department of Oral and Maxillofacial Pathology, Thai Moogambigai Dental College & Hospital, Chennai, India.
Journal of Pharmacy & Bioallied Sciences
|October 16, 2012
Summary
Immunohistochemistry (IHC) uses antibodies to map antigen distribution in tissues for disease diagnosis. This technique is crucial for identifying cancer markers in diagnostic and research settings.
Area of Science:
- Biomedical Sciences
- Pathology
- Immunology
Background:
- Immunohistochemistry (IHC) is a key laboratory technique utilizing antibody-based detection.
- It visualizes the specific distribution of antigens within biological tissues.
- This method is vital in both health and disease research and diagnostics.
Purpose of the Study:
- To review the diverse applications of Immunohistochemistry (IHC).
- To highlight the significance of IHC in disease diagnosis, particularly in oncology.
- To underscore the role of IHC in diagnostic and research laboratories.
Main Methods:
- Application of monoclonal and polyclonal antibodies.
- Detection of specific antigen localization in tissue samples.
- Utilizing IHC for diagnostic analysis of various diseases.
Main Results:
- Demonstrates the utility of IHC in identifying tissue-specific antigen distribution.
- Confirms the widespread use of IHC in cancer diagnosis due to tumor antigen expression.
- Highlights IHC's critical role in both diagnostic and research laboratory workflows.
Conclusions:
- Immunohistochemistry is an indispensable tool for disease diagnosis and research.
- The technique's ability to detect antigen distribution aids in understanding health and disease states.
- IHC plays a pivotal role in modern pathology and oncology diagnostics.
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