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Full-length synaptonemal complex grows continuously during meiotic prophase in budding yeast.

Karen Voelkel-Meiman1, Sarah S Moustafa, Philippe Lefrançois

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The synaptonemal complex (SC) in budding yeast continuously incorporates Zip1 proteins during meiosis. This dynamic assembly, even after initial formation, influences crossover recombination and chromosome pairing.

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Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • The synaptonemal complex (SC) is crucial for homologous chromosome pairing and crossover recombination during meiosis.
  • SC assembly involves proteins like Zip1, but its dynamic remodeling capacity after formation is unclear.

Purpose of the Study:

  • To investigate the dynamics of the full-length synaptonemal complex (SC) in budding yeast.
  • To understand the remodeling capacity and turnover of SC components, particularly Zip1, after initial assembly.

Main Methods:

  • Utilized budding yeast meiotic cells to study SC dynamics.
  • Employed mutant Zip1 versions (Zip1-4LA) to assess rescue capabilities.
  • Analyzed spatial distribution and incorporation patterns of Zip1.

Main Results:

  • Demonstrated continuous Zip1 incorporation into existing SC structures during meiotic prophase.
  • Showed that post-synapsis Zip1 incorporation can rescue sporulation defects caused by mutant Zip1.
  • Observed non-uniform Zip1 distribution, initially associated with Zip3, independent of synapsis initiation components or homolog presence.

Conclusions:

  • The SC central region exhibits limited global turnover post-assembly but maintains differential dynamics.
  • Zip1 incorporation dynamics are influenced by recombination landscape and Zip1 copy number.
  • SC remodeling is a regulated process occurring even after full-length assembly.