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Updated: May 17, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
Published on: September 15, 2021
Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction
Yasutsugu Tamura1, Kazuhiro Furukawa, Rei Yoshimoto
1Nano Medical Engineering Laboratory, RIKEN Advanced Science Insutitute, 2-1 Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
Abstract:
RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken δ-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process.
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