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Related Concept Videos

Xylem and Transpiration-driven Transport of Resources02:03

Xylem and Transpiration-driven Transport of Resources

The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
Phloem and Sugar Transport02:02

Phloem and Sugar Transport

Like many living organisms, plants have tissues that specialize in specific plant functions. For example, shoots are well adapted to rapid growth, while roots are structured to acquire resources efficiently. However, sugar production is primarily restricted to the photosynthetic cells that reside in the leaves of angiosperm plants. Sugar and other resources are transported from photosynthetic tissues to other specialized tissues by a process called translocation.

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Related Experiment Video

Updated: May 17, 2026

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
10:47

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope

Published on: June 20, 2019

Methods for xylem sap collection.

M Alexou1, A D Peuke

  • 1National Agricultural Research Foundation (NAGREF), Forest Research Institute (FRI), Thessaloniki, Greece.

Methods in Molecular Biology (Clifton, N.J.)
|October 18, 2012
PubMed
Summary
This summary is machine-generated.

Xylem and phloem are vital for plant transport. This study details methods for collecting xylem sap, crucial for understanding water, nutrient, and signal movement in plants.

More Related Videos

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
09:38

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

Published on: October 23, 2013

Related Experiment Videos

Last Updated: May 17, 2026

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope
10:47

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope

Published on: June 20, 2019

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
09:38

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

Published on: October 23, 2013

Area of Science:

  • Plant Physiology
  • Plant Biology
  • Biochemistry

Background:

  • Xylem and phloem facilitate essential solute and signal exchange among land plant organs.
  • This transport system is critical for distributing water, nutrients, and metabolic products throughout the plant.
  • Analyzing xylem sap provides insights into bulk transport within the transpiration stream.

Purpose of the Study:

  • To describe methods for collecting xylem sap.
  • To enable the quantification of element, ion, and compound flow by estimating volume flow.
  • To provide a comprehensive overview of xylem sap collection techniques.

Main Methods:

  • Root pressure exudate collection.
  • Scholander-Hammel pressure chamber technique.
  • Root pressurizing method (Passioura).
  • Vacuum pump method (hand/battery operated).

Main Results:

  • The study outlines four distinct methods for xylem sap collection.
  • These methods facilitate the qualitative and quantitative analysis of xylem transport.
  • Successful application of these methods allows for the assessment of bulk flow and solute movement.

Conclusions:

  • Accurate xylem sap collection is fundamental for understanding plant physiological processes.
  • The described methods offer versatile approaches for researchers studying plant transport.
  • Quantifying volume flow alongside sap analysis is key to a complete understanding of nutrient and signal transport.