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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: May 17, 2026

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Plant single cell sampling.

Wieland Fricke¹

¹School of Biology and Environmental Science, University College Dublin (UCD), Dublin, Ireland, Wieland02fricke@yahoo.co.uk.

Methods in Molecular Biology (Clifton, N.J.)

|October 18, 2012

Summary

This summary is machine-generated.

Single cell analysis in plants reveals nutrient distribution. Methods like cell sap sampling and picoliter osmometry help understand mineral nutrition and nutrient storage in plants.

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Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

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Last Updated: May 17, 2026

Isolation and Transcriptome Analysis of Plant Cell Types

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Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

Area of Science:

Plant Physiology
Analytical Chemistry
Biochemistry

Background:

Understanding plant mineral nutrition requires knowledge of nutrient distribution at the cellular level.
Plants adapt nutrient storage and mobilization based on external availability, necessitating precise analytical methods.

Purpose of the Study:

To detail single plant cell sampling and analysis methods for solute concentration determination.
To discuss the advantages and limitations of these techniques for mineral nutrition studies.

Main Methods:

Pressure-driven extraction of cell contents for cell sap sampling.
Picoliter osmometry for osmolality measurements.
Energy-dispersive X-ray (EDX) analysis for elemental concentrations (Na, K, P, S, Cl, Ca).
Microfluorometry for anion and amino acid concentrations.

Main Results:

The described methods enable the determination of solute concentrations in individual plant cells and tissues.
Extracted cell sap is predominantly of vacuolar origin.
These techniques provide insights into cell- and tissue-specific nutrient distribution.

Conclusions:

Single cell analysis is crucial for understanding plant mineral nutrition and nutrient dynamics.
Selected methods offer valuable tools for detailed investigation of solute concentrations in plants.
The vacuole plays a significant role in the storage of analyzed solutes.