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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
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UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

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Related Experiment Video

Updated: May 17, 2026

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
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Published on: October 11, 2019

Visible spectrophotometric method for amiodarone.

Andreea Bosînceanu1, Graţiela Popa, Gladiola Tântaru

  • 1Discipline of Pharmaceutical Technology, School of Pharmacy, University of Medicine and Pharmacy, "Grigore T. Popa", Iasi.

Revista Medico-Chirurgicala a Societatii De Medici Si Naturalisti Din Iasi
|October 20, 2012
PubMed
Summary
This summary is machine-generated.

A new spectrophotometric method accurately quantifies amiodarone, an antiarrhythmic drug. This method utilizes potassium ferricyanide reduction and achieves high accuracy within a specific concentration range.

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Area of Science:

  • Analytical Chemistry
  • Pharmacology

Background:

  • Amiodarone is a critical antiarrhythmic medication for managing ventricular and supraventricular tachyarrhythmias.
  • Accurate quantification of amiodarone is essential for therapeutic drug monitoring and quality control.

Purpose of the Study:

  • To establish a reliable spectrophotometric method for amiodarone assay.
  • To determine the optimal conditions and validation parameters for the developed method.

Main Methods:

  • A spectrophotometric assay was developed based on the reduction of potassium ferricyanide in hydrochloric acid.
  • The formation of a blue complex, ferric ferrocyanide, with Fe (III) ions was measured.
  • Optimal stability and maximum absorption were determined in an ethylic alcohol and water mixture (2:1, v/v) at 725 nm.

Main Results:

  • The method followed the Lambert-Beer Law in the concentration range of 0.5-5.0 microg/sample.
  • High linearity was observed with a correlation coefficient (R) of 0.99977 and R² of 0.999541.
  • The detection limit (DL) was 0.1032 microg/sample and the quantification limit (QL) was 0.344 microg/sample.

Conclusions:

  • The developed spectrophotometric method provides a sensitive and accurate means for amiodarone quantification.
  • This assay is suitable for routine laboratory analysis and quality control of amiodarone formulations.