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Updated: May 17, 2026

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography
Published on: October 25, 2021
Using cross-correlation for automated stitching of two-dimensional multi-tile electron backscatter diffraction data
A L Pilchak1, A R Shiveley, P A Shade
1Air Force Research Laboratory, Materials and Manufacturing Directorate / RXLM, Wright Patterson Air Force Base, Ohio, USA. adam.pilchak@wpafb.af.mil
Abstract:
A method for automatically aligning consecutive data sets of large, two-dimensional multi-tile electron backscatter diffraction (EBSD) scans with high accuracy was developed. The method involved first locating grain and phase boundaries within search regions containing overlapping data in adjacent scan tiles, and subsequently using cross-correlation algorithms to determine the relative position of the individual scan tiles which maximizes the fraction of overlapping boundaries. Savitzky-Golay filtering in two dimensions was used to estimate the background, which was then subtracted from the cross-correlation to enhance the peak signal in samples with a high density of interfaces. The technique was demonstrated on data sets with a range of interface densities. The equations were implemented as enhancements to a recently published open source code for stitching of multi-tile EBSD data sets.
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