Related Experiment Video
Updated: May 17, 2026
Gene Editing of Primary Rhesus Macaque B Cells
Published on: February 10, 2023
Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)
Genesio M Karere1, Erin Sullivan, Erin L Kinnally
1Department of Population Health and Reproduction, University of California-Davis, Davis, CA, USA. gmkarere@txbiomedgenetics.org
Background:
Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior.
Methods:
We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data.
Results:
Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective.
Conclusions:
Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
