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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Maltose-binding protein is a potential carrier for oral immunizations
P Bellot1, P Tiels, V Melkebeek
1Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Veterinary Immunology and Immunopathology
|October 20, 2012
Summary
Oral administration of Maltose binding protein (MBP) can induce immune responses in pigs. MBP may act as a carrier to deliver vaccine antigens to intestinal immune cells, enhancing their delivery.
Area of Science:
- Immunology
- Vaccinology
- Gastroenterology
Background:
- Maltose binding protein (MBP) enhances protein yield and purification.
- MBP may boost immunogenicity by stimulating dendritic cell maturation via TLR4.
- Investigating MBP's role in eliciting immune responses after oral administration is crucial.
Purpose of the Study:
- To determine if oral MBP administration elicits an MBP-specific immune response.
- To assess both systemic and mucosal immune responses following oral MBP immunization.
- To explore MBP's potential as a carrier for oral vaccines.
Main Methods:
- Oral immunization of piglets with MBP or MBP combined with cholera toxin (CT).
- Oral administration of MBP-fused protein (MBPFedF) with CT.
- Analysis of systemic and mucosal immune responses, including antibody titers and antibody-secreting cells (ASCs).
Main Results:
- Oral MBP induced a local mucosal IgM response in jejunal Peyer's patches, but not a high systemic response.
- MBPFedF+CT induced a significant systemic response against MBP and a weak response against FedF.
- MBP-specific IgA ASCs in the lamina propria indicated a local intestinal immune response against MBP.
Conclusions:
- Maltose binding protein can traverse the intestinal epithelial barrier after oral administration in pigs.
- MBP can reach gut-associated lymphoid tissues, suggesting its potential as a carrier for oral vaccine antigens.
- MBP may facilitate targeted delivery of vaccine antigens to intestinal immune cells.
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