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Related Experiment Video

Updated: May 17, 2026

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
11:44

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Published on: March 24, 2015

Circulating neutrophil dysfunction in acute liver failure.

Nicholas J Taylor1, Anirudh Nishtala, Godhev K Manakkat Vijay

  • 1Liver Intensive Care Unit and Transplantation, King's College London School of Medicine at King's College Hospital, London, UK; Institute of Liver Studies and Transplantation, King's College London School of Medicine at King's College Hospital, London, UK.

Hepatology (Baltimore, Md.)
|October 20, 2012
PubMed
Summary
This summary is machine-generated.

Neutrophil function is impaired in acute liver failure (ALF) and subacute liver failure (SALF), similar to severe sepsis. This impaired neutrophil phagocytic activity predicts poor outcomes in ALF/SALF patients.

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Area of Science:

  • Hepatology
  • Immunology
  • Critical Care Medicine

Background:

  • Acute liver failure (ALF) and subacute liver failure (SALF) are associated with systemic inflammation and increased sepsis susceptibility.
  • Neutrophil dysfunction is implicated in organ damage and failure during ALF/SALF.

Purpose of the Study:

  • To characterize the function of circulating neutrophils in patients with ALF and SALF.
  • To assess neutrophil phenotype, phagocytic activity, and oxidative burst in ALF/SALF patients.
  • To determine if neutrophil function predicts outcomes in ALF/SALF.

Main Methods:

  • Prospective study of 25 ALF/SALF patients, compared with healthy controls (HC) and septic controls (SC).
  • Neutrophil isolation and analysis of CD16 and CD11b expression via flow cytometry.
  • Assessment of neutrophil phagocytic activity (NPA) using E. coli and oxidative burst (OB) via ROS production.

Main Results:

  • Reduced neutrophil CD16 expression in ALF patients compared to HC.
  • Significantly impaired NPA in SALF patients compared to HC.
  • Impaired NPA on admission predicted non-survival without liver transplantation in ALF/SALF cohorts.
  • Stimulated oxidative burst was preserved in ALF/SALF but impaired in septic controls.

Conclusions:

  • Circulating neutrophils in ALF/SALF exhibit impaired bactericidal function, mirroring severe sepsis.
  • Neutrophil function indices serve as critical biomarkers in ALF, potentially contributing to organ dysfunction and sepsis susceptibility.