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Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer
Jinyu Wang1, Jean-François Léger, Jonas Binding
1Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, F-75005 France ; Inserm, U1024, Paris F-75005 France ; CNRS, UMR 8197, Paris, F-75005 France ; Institut Langevin, ESPCI ParisTech, CNRS UMR 7587, ESPCI, 1 rue Jussieu, 75005 Paris, France ; Fondation Pierre-Gilles de Gennes pour la Recherche, 29 rue d'Ulm, Paris, 75005 France.
Abstract:
Aberrations limit the resolution, signal intensity and achievable imaging depth in microscopy. Coherence-gated wavefront sensing (CGWS) allows the fast measurement of aberrations in scattering samples and therefore the implementation of adaptive corrections. However, CGWS has been demonstrated so far only in weakly scattering samples. We designed a new CGWS scheme based on a Linnik interferometer and a SLED light source, which is able to compensate dispersion automatically and can be implemented on any microscope. In the highly scattering rat brain tissue, where multiply scattered photons falling within the temporal gate of the CGWS can no longer be neglected, we have measured known defocus and spherical aberrations up to a depth of 400 µm.
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