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Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik interferometer.

Jinyu Wang1, Jean-François Léger, Jonas Binding

  • 1Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, F-75005 France ; Inserm, U1024, Paris F-75005 France ; CNRS, UMR 8197, Paris, F-75005 France ; Institut Langevin, ESPCI ParisTech, CNRS UMR 7587, ESPCI, 1 rue Jussieu, 75005 Paris, France ; Fondation Pierre-Gilles de Gennes pour la Recherche, 29 rue d'Ulm, Paris, 75005 France.

Biomedical Optics Express
|October 20, 2012
PubMed
Summary

This study introduces a new coherence-gated wavefront sensing (CGWS) method for microscopy. The technique successfully measures aberrations in highly scattering biological tissues, improving imaging depth.

Keywords:
(010.7350) Wave-front sensing(110.0113) Imaging through turbid media(110.1080) Active or adaptive optics

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Area of Science:

  • Optical microscopy
  • Biomedical optics
  • Adaptive optics

Background:

  • Microscopy aberrations limit resolution, signal intensity, and imaging depth.
  • Coherence-gated wavefront sensing (CGWS) enables aberration measurement and correction in scattering samples.
  • Previous CGWS methods were limited to weakly scattering samples.

Purpose of the Study:

  • To develop and demonstrate a novel CGWS scheme for aberration correction in highly scattering biological tissues.
  • To overcome the limitations of existing CGWS techniques in complex scattering environments.
  • To enable deeper and clearer imaging in biological samples.

Main Methods:

  • A new CGWS scheme was designed using a Linnik interferometer and a superluminescent diode (SLED) light source.
  • The system automatically compensates for dispersion.
  • The method was implemented on a standard microscope setup.

Main Results:

  • The new CGWS scheme was successfully applied to highly scattering rat brain tissue.
  • Defocus and spherical aberrations were measured up to an imaging depth of 400 µm.
  • The method accounts for multiply scattered photons within the temporal gate.

Conclusions:

  • The developed CGWS technique extends aberration correction to highly scattering samples, a significant advancement for microscopy.
  • This method allows for improved imaging performance in challenging biological tissues.
  • The system's adaptability to any microscope facilitates broader application in biological research.