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Related Concept Videos

Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Chromatographic Resolution01:15

Chromatographic Resolution

In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Robust algorithm for aligning two-dimensional chromatograms.

Jonas Gros1, Deedar Nabi, Petros Dimitriou-Christidis

  • 1Environmental Chemistry Modeling Laboratory (LMCE), GR C2 544, Swiss Federal Institute of Technology at Lausanne (EPFL), Station 2, CH-1015 Lausanne, Switzerland.

Analytical Chemistry
|October 23, 2012
PubMed
Summary
This summary is machine-generated.

A new algorithm for aligning comprehensive two-dimensional gas chromatography (GC × GC) data improves accuracy and minimizes signal modification. This method is valuable for analyzing complex mixtures in environmental forensics and weathering studies.

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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
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Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector
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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Comprehensive two-dimensional gas chromatography (GC × GC) analysis often shows run-to-run retention time variability.
  • Chromatogram alignment is crucial for accurate data analysis in fields like environmental forensics.

Purpose of the Study:

  • To develop and evaluate a novel algorithm for aligning entire GC × GC chromatograms.
  • To compare the new algorithm's performance against existing methods.

Main Methods:

  • The algorithm uses user-defined alignment points in target and reference chromatograms.
  • Applied to oil spill and wastewater extract samples with varying analytical conditions.

Main Results:

  • The new algorithm demonstrated superior performance compared to two other alignment techniques.
  • Achieved better retention time matching for analytes and reduced artifacts.
  • Minimized modification of quantitative signal information.

Conclusions:

  • The developed algorithm offers an effective solution for GC × GC chromatogram alignment.
  • Provides improved accuracy and data integrity for complex mixture analysis.