Fluorescent nanoparticles for intracellular sensing: a review

Area of Science:

• Nanotechnology
• Biomedical Engineering
• Analytical Chemistry

Background:

• Fluorescent nanoparticles (NPs) have gained prominence over the last decade.
• They offer advantages like enhanced fluorescence, photostability, and biocompatibility over organic dyes.
• Their high surface-to-volume ratio enables functionalization for sensing applications.

Purpose of the Study:

• To review the developments and analytical applications of fluorescent NPs for intracellular chemical and biological sensing.
• To highlight the potential of fluorescent NPs in fluorescence lifetime imaging microscopy (FLIM).
• To provide an overview of current cell delivery methods for fluorescent NPs.

Main Methods:

• Literature review focusing on fluorescent nanoparticle research.
• Analysis of applications in intracellular sensing and imaging.
• Examination of nanoparticle delivery strategies into cells.

Main Results:

• Fluorescent NPs are versatile tools for intracellular monitoring.
• They show significant potential for advanced imaging techniques like FLIM.
• Various cell delivery methods exist, each with distinct advantages and disadvantages.

Conclusions:

• Fluorescent nanoparticles are powerful tools for biomedical research, particularly for intracellular sensing and imaging.
• Further research into their development and delivery is crucial for maximizing their potential.
• Their application in FLIM and other advanced microscopy techniques is a promising area for future exploration.