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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: May 17, 2026

Electron Cryotomography of Bacterial Cells
14:23

Electron Cryotomography of Bacterial Cells

Published on: May 6, 2010

Cellular nanoimaging by cryo electron tomography.

Roman I Koning1, Abraham J Koster

  • 1Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands. r.i.koning@lumc.nl

Methods in Molecular Biology (Clifton, N.J.)
|October 23, 2012
PubMed
Summary
This summary is machine-generated.

Cryo electron tomography provides 3D visualization of cells at nanometer resolution, revealing cellular structures and macromolecular arrangements. This technique offers direct molecular-level insight into the cellular environment.

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Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Published on: November 20, 2021

Area of Science:

  • Structural Biology
  • Cell Biology
  • Biophysics

Background:

  • Cryo electron tomography (CET) enables high-resolution 3D imaging of biological samples.
  • It visualizes cellular morphology, membranous structures, and macromolecular arrangements.
  • CET is unique for direct visualization of cellular space at molecular resolution.

Purpose of the Study:

  • To present laboratory methods for performing cellular cryo electron tomography.
  • To detail instrumentation, materials, and protocols for whole-cell CET.
  • To outline the multidisciplinary expertise required for successful CET.

Main Methods:

  • Cell growth and preparation for cryo immobilization.
  • Specimen vitrification and electron microscopy techniques.
  • Tomographic image reconstruction and 3D visualization.

Main Results:

  • Established protocols for cellular cryo electron tomography.
  • Demonstrated visualization of cellular morphology and internal structures.
  • Provided a comprehensive guide to CET methodology.

Conclusions:

  • Cellular cryo electron tomography is a powerful tool for structural biology.
  • The presented methods facilitate detailed molecular-level analysis of whole cells.
  • Expertise in cell biology, physics, and image processing is crucial for CET.