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Related Concept Videos

The Blood-brain Barrier00:49

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Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
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Related Experiment Video

Updated: May 17, 2026

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics
08:43

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Published on: November 30, 2012

Translocating the blood-brain barrier using electrostatics.

Marta M B Ribeiro1, Marco M Domingues, João M Freire

  • 1Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa Lisboa, Portugal.

Frontiers in Cellular Neuroscience
|October 23, 2012
PubMed
Summary
This summary is machine-generated.

Mammalian cell membranes, particularly at the blood-brain barrier, exhibit significant negative charge. This anionicity influences how compounds cross into the brain, impacting drug delivery and transport.

Keywords:
blood cellsblood-brain barriercell surface chargedrug targetingzeta-potential

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Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

Area of Science:

  • Biochemistry
  • Cell Biology
  • Neuroscience

Background:

  • Mammalian cell membranes are crucial for cellular functions, with surface charge influencing key events.
  • Quantitative data on membrane charge and its role in pharmacokinetics/pharmacodynamics is limited.
  • Previous work indicated brain endothelial cells possess greater anionicity than umbilical cord cells.

Purpose of the Study:

  • To investigate the hypothesis that anionicity is a key feature of the blood-brain barrier (BBB).
  • To determine if BBB anionicity contributes to the selective passage of compounds into the brain.
  • To compare the membrane surface charge of brain cells with various blood components.

Main Methods:

  • Zeta-potential measurements using dynamic light scattering to assess cell membrane surface charge.
  • Evaluation of model membranes with varying anionic lipid percentages to correlate zeta-potential with charge density.
  • Comparative analysis of brain endothelial cells, red blood cells, platelets, and peripheral blood mononuclear cells.

Main Results:

  • Brain endothelial cells exhibit the highest degree of anionicity among all tested mammalian cells.
  • Blood components like red blood cells, platelets, and PBMCs show less negative surface charges compared to brain cells.
  • Model membranes demonstrated a direct correlation between negative zeta-potential and increased anionic lipid content.

Conclusions:

  • The blood-brain barrier possesses a significantly anionic surface charge, distinguishing it from other cell types.
  • This pronounced anionicity is a critical factor in the selective transport of substances across the BBB.
  • Lipophilic cationic compounds are more likely to permeate the blood-brain barrier due to these electrostatic interactions.